https://syncellwiki.org/wiki/index.php/Special:ExportRDF/Lavickova2020_-2D_Partially_Self-2DRegenerating_Synthetic_Cell
type: Ontology
creation date: 2026-08-25T09:55:49-07:00
imports: http://semantic-mediawiki.org/swivt/1.0

Lavickova2020 - Partially Self-Regenerating Synthetic Cell
type: Subject
type: Category:LiteratureEntry
page: https://syncellwiki.org/wiki/index.php/Lavickova2020_-2D_Partially_Self-2DRegenerating_Synthetic_Cell
wiki namespace: 0
wiki page content language: en
has abstract: The reconstruction of biological processes in vitro offers a powerful route towards the bottom-up construction of synthetic cells. However, the complex dynamics of biological systems make their emulation in synthetic systems a considerable challenge. Here, we present a partially self-regenerating minimal cell consisting of a microfluidic reactor hosting a cell-free gene expression system, which is periodically replenished with small volumes of feeding solution. This system sustains the expression of several essential protein components, including ribosomal proteins, translation factors, and T7 RNA polymerase, over a 24-h period. By maintaining a balance between supply and removal, and by avoiding energy depletion and component degradation, the system exhibits an increase in self-regeneration capacity. Our work demonstrates a step towards the sustained operation of synthetic minimal cells.
has authors: Barbora Lavickova, Nadanai Laohakunakorn, Sebastian J. Maerkl
has DOI: s41467-020-20180-6
has e cell-freeperimental conte cell-freet: Cell-free
has source: Nature Communications
has subsystem tag: Metabolic Subsystem
has title: A partially self-regenerating synthetic cell
has year: 2020
wiki page modification date: 2025-08-15T11:27:41Z
modification date#aux: 2460902.9775579
wiki page sort key: Lavickova2020 - Partially Self-Regenerating Synthetic Cell